The online FRAST tool is also part of a collective fungal genomics software toolkit called MycoProcessor, that has been co-developed by CCDM and AAGI. MycoProcessor applies FRAST to pangenomic data to identify known fungicide resistance mutations in fungal pathogens at a larger scale, to support fungicide resistance monitoring and surveillance. By analysing this ‘pathogenomic’ data, MycoProcessor and FRAST can help researchers identify resistance risks before they become widespread problems in the field.
FRAST works by aligning user supplied fungal protein (amino acid) sequences to curated reference “archetype” proteins, and automatically identifies known target site mutations associated with fungicide resistance. Fungicide resistance research literature can be confusing due to different mutation positions being reported across different species, however FRAST removes this ambiguity by reporting detected resistance mutations using a standardised nomenclature, with results available as sequence alignments or downloadable tables.
Developed by researchers at the Centre for Crop and Disease Management, FRAST can be readily integrated into crop disease research and pathogen genomics workflows.
